Difference between revisions of "Part:BBa K1114000:Design"

Revision as of 18:25, 7 September 2013

The MoClo format of BBa_J23100 with AB fusion sites.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part.

This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=”https://parts.igem.org/Part:BBa_K1114102“>BBa_K783055/a>.

</html>

Source

iGEM Distribution Kit

References

Source

iGEM Distribution Kit

@@ Line 4: / Line 4: @@
 <partinfo>BBa_K1114000 SequenceAndFeatures</partinfo>
@@ Line 11: / Line 14: @@
 </html>
-Summary of Modifications from original part:
-Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
+This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
+The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
+Summary of modifications from original part:
+Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K1114102“>BBa_K783055/a>.
+</html>
+===Source===
+iGEM Distribution Kit
+===References===
 ===Source===