Difference between revisions of "Part:BBa K1216006"
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Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives | Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives | ||
− | [[File:Acetyl_esterase_3D.jpg|thumb| 3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB] ]] | + | [[File:Acetyl_esterase_3D.jpg|thumb| 3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB] ]] The poly-HIS tag can be used for protein purification (IMAC)[[Part:BBa_K1216001#References|<sup>[1]</sup>]]. The TEV tag can then be used to have the TEV protease specifically cleave off the poly-HIS tag from the purified protein [[Part:BBa_K1216001#References|<sup>[2]</sup>]]. |
− | A form of this protein | + | A form of this protein without TEV and poly-HIS tags can be found [[Part:BBa_K1216002| here]]. |
<!-- Add more about the biology of this part here --> | <!-- Add more about the biology of this part here --> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[[Part:BBa_K1216002#References|<sup>[ | + | The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[[Part:BBa_K1216002#References|<sup>[3]</sup>]] . Further it can be used for desacteylation of β-Lactam antibiotics[[Part:BBa_K1216002#References|<sup>[3]</sup>]] . |
− | The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[[Part:BBa_K1216002#References|<sup>[ | + | The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[[Part:BBa_K1216002#References|<sup>[4]</sup>]] [[Part:BBa_K1216002#References|<sup>[5]</sup>]]. |
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===References=== | ===References=== | ||
+ | # Loghran ST, "Purification of poly-histidine-tagged proteins.",Methods Mol Biol. 2011;681:311-35. doi: 10.1007/978-1-60761-913-0_17.[http://www.ncbi.nlm.nih.gov/pubmed/20978973] | ||
+ | # [http://homepage.univie.ac.at/nikos.pinotsis/tev_protease.html University of Vienna TEV Protease info] | ||
# [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech] | # [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech] | ||
# [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc] | # [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc] | ||
# [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc] | # [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc] |
Revision as of 13:11, 6 September 2013
Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags
Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives
The poly-HIS tag can be used for protein purification (IMAC)[1]. The TEV tag can then be used to have the TEV protease specifically cleave off the poly-HIS tag from the purified protein [2].A form of this protein without TEV and poly-HIS tags can be found here.
Usage and Biology
The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[3] . Further it can be used for desacteylation of β-Lactam antibiotics[3] .
The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[4] [5].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 951
References
- Loghran ST, "Purification of poly-histidine-tagged proteins.",Methods Mol Biol. 2011;681:311-35. doi: 10.1007/978-1-60761-913-0_17.[http://www.ncbi.nlm.nih.gov/pubmed/20978973]
- [http://homepage.univie.ac.at/nikos.pinotsis/tev_protease.html University of Vienna TEV Protease info]
- [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
- [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
- [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]