Difference between revisions of "Part:BBa V1001:Design"
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===Design Notes===
 
===Design Notes===
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Genotype:  
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[http://openwetware.org/wiki/E._coli_genotypes Genotype]:  
−
 
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−
F-φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR, recA1 endA1 hsdR17(rk- mk+ phoA supE44 λ- thi-1 gyrA96 relA1
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−
 
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−
 
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F-&#966;80dlacZ&#916;M15 &#916;(lacZYA-argF)U169 deoR, recA1 endA1 hsdR17(rk- mk+ phoA supE44 &#955;- thi-1 gyrA96 relA1      <small>[http://openwetware.org/wiki/E._coli_genotypes What does this mean (?)] </small>
  
 
===Source===
 
===Source===
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* <I>E.coli</I> Genetic Stock Center (CGSC) at Yale University
 
* <I>E.coli</I> Genetic Stock Center (CGSC) at Yale University
 
<A HREF='http://cgsc.biology.yale.edu'>CGSC</A>
 
<A HREF='http://cgsc.biology.yale.edu'>CGSC</A>
 +
*FOCUS (1986) 8:2, 9.
 +
*Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
 +
*Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
 +
* Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.

Latest revision as of 20:13, 25 June 2006


DH5a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[http://openwetware.org/wiki/E._coli_genotypes Genotype]:

F-φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR, recA1 endA1 hsdR17(rk- mk+ phoA supE44 λ- thi-1 gyrA96 relA1 [http://openwetware.org/wiki/E._coli_genotypes What does this mean (?)]

Source

References

  • E.coli Genetic Stock Center (CGSC) at Yale University

<A HREF='http://cgsc.biology.yale.edu'>CGSC</A>

  • FOCUS (1986) 8:2, 9.
  • Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
  • Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
  • Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.