Difference between revisions of "Part:BBa K1216001"
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The alkaline phosphatase is a periplasmic homodimeric hydrolase. Each monomer contains 429 amino acids.[[File:Alkaline_Phosphatase_3D.jpg|thumb| 3D representation of the alkaline phosphatase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=1ANJ RCSB] ]]  
 
The alkaline phosphatase is a periplasmic homodimeric hydrolase. Each monomer contains 429 amino acids.[[File:Alkaline_Phosphatase_3D.jpg|thumb| 3D representation of the alkaline phosphatase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=1ANJ RCSB] ]]  
  
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A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216004| here]].
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A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216005| here]].
  
 
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Revision as of 08:38, 2 September 2013

Alkaline Phosphatase (phoA) from Citrobacter

The alkaline phosphatase is a periplasmic homodimeric hydrolase. Each monomer contains 429 amino acids.
3D representation of the alkaline phosphatase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=1ANJ RCSB]

A form of this protein with added TEV and poly-HIS tags can be found here.

Usage and Biology

Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[1]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[2].

Alkaline phosphatases cleave phosphate groups from organic compounds by hydrolysis while retaining stereochemistry[3]. A good explanation of the mechanism can be found [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates here].
Alkaline phosphatases, respectively their serum levels, are also related to several diseases e.g. metabolic myopathies and Paget Disease. [4]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004.
  2. [http://www.nlm.nih.gov/medlineplus/ency/article/003470.htm Medline Plus]
  3. [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates Section 10.3: Hydrolysis of phosphates]
  4. Adams & Victor's Principles Of Neurology, 7th edition, McGraw-Hill Professional, 2000.