Difference between revisions of "Part:BBa K1216001"

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<partinfo>BBa_K1216001 short</partinfo>
 
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The alkaline phosphatase is a periplasmic homodimeric hydrolase.
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The alkaline phosphatase is a periplasmic homodimeric hydrolase. Each monomer contains 429 amino acids.
  
 
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Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [[Part:BBa_K1216001#References|<sup>[1]</sup>]]  
 
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [[Part:BBa_K1216001#References|<sup>[1]</sup>]]  
  
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Alkaline phosphatases, respectively their serum levels, are also related to several diseases e.g. metabolic myopathies and Paget Disease. [[Part:BBa_K1216001#References|<sup>[2]</sup>]]
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Alkaline phosphatases cleave phosphate groups from organic compounds by hydrolysis while retaining stereochemistry[[Part:BBa_K1216001#References|<sup>[2]</sup>]].
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A good explanation of the mechanism can be found [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates here].<br>
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Alkaline phosphatases, respectively their serum levels, are also related to several diseases e.g. metabolic myopathies and Paget Disease. [[Part:BBa_K1216001#References|<sup>[3]</sup>]]
  
 
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===References===
 
===References===
 
# Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004.
 
# Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004.
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# [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates Section 10.3: Hydrolysis of phosphates]
 
# Adams & Victor's Principles Of Neurology, 7th edition, McGraw-Hill Professional, 2000.
 
# Adams & Victor's Principles Of Neurology, 7th edition, McGraw-Hill Professional, 2000.

Revision as of 06:46, 2 September 2013

Alkaline Phosphatase (phoA) from Citrobacter

The alkaline phosphatase is a periplasmic homodimeric hydrolase. Each monomer contains 429 amino acids.

Usage and Biology

Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [1]

Alkaline phosphatases cleave phosphate groups from organic compounds by hydrolysis while retaining stereochemistry[2]. A good explanation of the mechanism can be found [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates here].
Alkaline phosphatases, respectively their serum levels, are also related to several diseases e.g. metabolic myopathies and Paget Disease. [3]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004.
  2. [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates Section 10.3: Hydrolysis of phosphates]
  3. Adams & Victor's Principles Of Neurology, 7th edition, McGraw-Hill Professional, 2000.