Difference between revisions of "Part:BBa K1123015:Design"
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===Design Notes=== | ===Design Notes=== | ||
The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1PJN protein sequence was repeated 5 times. | The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1PJN protein sequence was repeated 5 times. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 13:35, 31 August 2013
DNA Coding Sequence for 1PJN Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1PJN protein sequence was repeated 5 times.
Source
The amino acid sequence for this parts was retrieved from PDB.org after a database query was performed looking for proteins with high Arginine and Lysine contents.