Difference between revisions of "Part:BBa K1218011:Design"
Sophia liang (Talk | contribs) (→References) |
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===Source=== | ===Source=== | ||
| − | + | CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al. | |
===References=== | ===References=== | ||
[[http://nar.oxfordjournals.org/content/41/15/7429]] | [[http://nar.oxfordjournals.org/content/41/15/7429]] | ||
Revision as of 20:00, 29 August 2013
Cas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1642
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3921
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4863
Illegal BsaI.rc site found at 4840
Design Notes
Mutagenesis to remove internal EcoRI site
Source
CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al.