Difference between revisions of "Part:BBa K1185002:Design"

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===Design Notes===
 
===Design Notes===
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There are no illegal restriction sites in this BioBrick. As the ''hbs'' gene is found naturally in ''B. subtilis'' there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein.
+
There are no illegal restriction sites in this BioBrick. As the ''hbs'' gene is found naturally in ''B. subtilis'' there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein. We have included a three frame stop codon at the end of our construct.
  
 
===Source===
 
===Source===

Revision as of 11:53, 29 August 2013


HBsu-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 881
    Illegal AgeI site found at 993
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no illegal restriction sites in this BioBrick. As the hbs gene is found naturally in B. subtilis there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein. We have included a three frame stop codon at the end of our construct.

Source

The source of the HBsu coding sequence was the B. subtilis strain 168 genome, SwissProtTM accession number: P08821. The RFP sequence was sourced from BBa_E1010 and the linker from: BBa_K105012.


References