Difference between revisions of "Part:BBa K1041000"

(→‎Pictures)
(→‎Pictures)
Line 13: Line 13:
 
==Pictures==
 
==Pictures==
 
<gallery>
 
<gallery>
−
Image:WP 000051.jpg|BBa_J04450 and BBa_K1041000 visualized under non-UV lightbox
+
Image:WP 000051.jpg|(Left) BBa_K1041000 and (right) BBa_J04450 visualized under non-UV lightbox
−
 
+
  
 +
Both parts BBa_K1041000 and BBa_J04450 have colonies that appear red under natural light. The addition of a NdeI site has not effected the ability for the RFP gene to be transcribed.
  
  
 
<partinfo>BBa_K1041000 parameters</partinfo>
 
<partinfo>BBa_K1041000 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 11:13, 24 August 2013

RFP Coding Device

Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]

Pictures

  • (Left) BBa_K1041000 and (right) BBa_J04450 visualized under non-UV lightbox

  • Both parts BBa K1041000 and BBa J04450 have colonies that appear red under natural light. The addition of a NdeI site has not effected the ability for the RFP gene to be transcribed.