Difference between revisions of "Part:BBa K1065104:Design"

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===Design Notes===
 
===Design Notes===
 
The gene sequence was extracted using the following primers:
 
The gene sequence was extracted using the following primers:
 +
  
 
>Primer Forward: prefix + RBS + spacer + ATG...
 
>Primer Forward: prefix + RBS + spacer + ATG...
 +
 
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
 
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
  
 
>Primer Reverse: sequence_TAATAA_suffix
 
>Primer Reverse: sequence_TAATAA_suffix
−
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
 
−
 
  
 +
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
  
 
===Source===
 
===Source===
  
−
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.
+
SAM synthetase gene sequence extracted by PCR from ''E. coli'' MG1655 strain genome.
  
 
===References===
 
===References===
 +
<html>
 +
<ol>
 +
<li>
 +
<a id="ref1"></a>
 +
<u>Ethylene biosynthesis and its regulation in higher plants</u><br/>
 +
Yang, S. F.; Hoffman N. E. <i>Ann. Rev. Plant Physiol.</i> <b>1965</b>, <i>35</i>, 155-89.
 +
</li>
 +
<li>
 +
<a id="ref2"></a>
 +
MetaCyc EC 2.5.1.6, <i>MetaCyc</i>. August 15th, 2013, from <a href="http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=S-ADENMETSYN-RXN">link</a>
 +
</li>
 +
<li>
 +
<a id="ref3"></a>
 +
<u>Ethylene biosynthesis and signaling networks</u><br/>
 +
Wang, K. L.-C.; Li, H.; Ecker J. R. <i>Plant Cell</i> <b>2002</b>, <i>Supplement</i>, S131-S151
 +
</li>
 +
</ol>
 +
</html>

Latest revision as of 08:48, 15 August 2013


RBS + SAM synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene sequence was extracted using the following primers:


>Primer Forward: prefix + RBS + spacer + ATG...

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

>Primer Reverse: sequence_TAATAA_suffix

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

Source

SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References

  1. Ethylene biosynthesis and its regulation in higher plants
    Yang, S. F.; Hoffman N. E. Ann. Rev. Plant Physiol. 1965, 35, 155-89.
  2. MetaCyc EC 2.5.1.6, MetaCyc. August 15th, 2013, from link
  3. Ethylene biosynthesis and signaling networks
    Wang, K. L.-C.; Li, H.; Ecker J. R. Plant Cell 2002, Supplement, S131-S151