Difference between revisions of "Part:BBa K1041001"

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<partinfo>BBa_K1041001 short</partinfo>
 
<partinfo>BBa_K1041001 short</partinfo>
  
This part contains the promoter sequence AntAgp, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene.  
+
This part contains the promoter sequence AntAgp, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:36, 9 August 2013

Neomycin Resistance Coding Device + AntG promoter

This part contains the promoter sequence AntAgp, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 816