Difference between revisions of "Part:BBa K1041001"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1041001 short</partinfo>
 
<partinfo>BBa_K1041001 short</partinfo>
  
−
Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.
+
This part contains the promoter sequence AntAgp, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:34, 9 August 2013

Neomycin Resistance Coding Device + AntG promoter

This part contains the promoter sequence AntAgp, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 816