Difference between revisions of "Part:BBa K1159003"
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<partinfo>BBa_K1159003 short</partinfo>
 
<partinfo>BBa_K1159003 short</partinfo>
  
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<html><table border="1" cellspacing="0" width="100%" !important><tr><td colspan="400" width="40%"><strong>BioBrick: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_">BBa_</a></strong></td><td colspan="600" width="60%"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> version 1.0</strong></td></tr><tr><td colspan="100" width="10%"><strong>RFC standard:</strong></td><td colspan="600" width="60%">RFC 25 BioBrick, so ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.</td><td colspan="300" width="30%">ORF from -8 to 1260 (excluding stop-codon)</td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;<u><i>ATGGCCGGC</i>AGGTTCGAA&nbsp;...&nbsp;GTTTATGAA<i>ACCGGT</i></u></span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MAGRFEEWVKDKHIPFKLNHPDDNYDDFKPLRKIIGDTRVVALGENSHFIKEFFLLRHTLLRFFIEDLGFTTFAFEFGFAEGQIINNWIHGQGTDDEIGR<br>101&nbsp;FLKHFYYPEELKTTFLWLREYNKAAKEKITFLGIDIPRNGGSYLPNMEIVHDFFRTADKEALHIIDDAFNIAKKIDYFSTSQAALNLHELTDSEKCRLTS<br>201&nbsp;QLARVKVRLEAMAPIHIEKYGIDKYETILHYANGMIYLDYNIQAMSGFISGGGMQGDMGAKDKYMADSVLWHLKNPQSEQKVIVVAHNAHIQKTPILYDG<br>301&nbsp;FLSCLPMGQRLKNAIGDDYMSLGITSYSGHTAALYPEVDTKYGFRVDNFQLQEPNEGSVEKAISGCGVTNSFVFFRNIPEDLQSIPNMIRFDSIYMKAEL<br>401&nbsp;EKAFDGIFQIEKSSVSEVVYETG<br></span></td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">27 (6.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">3 (0.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">15 (3.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">12 (2.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">19 (4.5%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">16 (3.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">14 (3.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">36 (8.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">31 (7.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">4 (0.9%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">20 (4.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">30 (7.1%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">34 (8.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">14 (3.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">19 (4.5%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">29 (6.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">31 (7.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">29 (6.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">22 (5.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">18 (4.3%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number:</td><td colspan="100" width="10.0%">423</td><td colspan="200" width="20.0%">Positively charged (Arg + Lys):</td><td colspan="100" width="10.0%">45 (10.6%)</td><td colspan="200" width="20.0%">Negatively charged (Asp + Glu):</td><td colspan="100" width="10.0%">59 (13.9%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular mass [Da]:</td><td colspan="100" width="10.0%">48459.2</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.55</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">50310 / 50497.5<br>(all Cys red/ox)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life [h]:</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">>20</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">30</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage (CAI):</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">good (0.74)</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">good (0.75)</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">good (0.66)</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table><br></html>
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<table border="1" cellspacing="0" width="100%" !important><tr><td colspan="1000"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> Version 1.0</strong></td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;GTACACA<u>ATGCGTCGT&nbsp;...&nbsp;TTCGAAAAA</u>TAA</span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE<br>101&nbsp;FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW<br>201&nbsp;FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL<br>301&nbsp;DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT<br>401&nbsp;ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE<br>501&nbsp;FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK<br></span></td></tr><tr><td colspan="200" width="20%"><strong>BioBrick: <partinfo>BBa_K801060</partinfo></strong></td><td colspan="800" width="80%">Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).</td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">33 (5.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">4 (0.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">18 (3.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">16 (2.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">26 (4.6%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">25 (4.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">24 (4.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">39 (6.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">28 (4.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">14 (2.5%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">27 (4.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">48 (8.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">64 (11.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">17 (3.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">27 (4.8%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">34 (6.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">29 (5.1%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">36 (6.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">33 (5.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">24 (4.2%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number of amino acids (aa):</td><td colspan="100" width="10.0%">566</td><td colspan="200" width="20.0%">Number of positively charged aa (Arg + Lys):</td><td colspan="100" width="10.0%">61</td><td colspan="200" width="20.0%">Number of negatively charged aa (Asp + Glu):</td><td colspan="100" width="10.0%">82</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular weight [Da]:</td><td colspan="100" width="10.0%">66105.33</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.38</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">117480 (all Cys as cystine)<br>117230 (all Cys reduced)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life:</strong></td><td colspan="200" width="20.0%"><i>Mammals:</i></td><td colspan="100" width="10.0%">30 h</td><td colspan="200" width="20.0%"><i>Yeast:</i></td><td colspan="100" width="10.0%">>20 h</td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10 h</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage:</strong> (CAI)</td><td colspan="200" width="20.0%"><i>Mammals:</i></td><td colspan="100" width="10.0%">0.68</td><td colspan="200" width="20.0%"><i>Yeast:</i></td><td colspan="100" width="10.0%">0.69</td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">0.71</td></tr><tr><td colspan="200" width="20.0%"><strong>RFC standard:</strong></td><td colspan="800" width="80.0%">This is a RFC 10 BioBrick, nothing was added</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:20, 28 July 2013

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

BioBrick: BBa_Automatically determined parameters using the BioBrick-AutoAnnotator version 1.0
RFC standard:RFC 25 BioBrick, so ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.ORF from -8 to 1260 (excluding stop-codon)
Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
 ATGGCCGGCAGGTTCGAA ... GTTTATGAAACCGGT
Amino acid sequence:
  1 MAGRFEEWVKDKHIPFKLNHPDDNYDDFKPLRKIIGDTRVVALGENSHFIKEFFLLRHTLLRFFIEDLGFTTFAFEFGFAEGQIINNWIHGQGTDDEIGR
101 FLKHFYYPEELKTTFLWLREYNKAAKEKITFLGIDIPRNGGSYLPNMEIVHDFFRTADKEALHIIDDAFNIAKKIDYFSTSQAALNLHELTDSEKCRLTS
201 QLARVKVRLEAMAPIHIEKYGIDKYETILHYANGMIYLDYNIQAMSGFISGGGMQGDMGAKDKYMADSVLWHLKNPQSEQKVIVVAHNAHIQKTPILYDG
301 FLSCLPMGQRLKNAIGDDYMSLGITSYSGHTAALYPEVDTKYGFRVDNFQLQEPNEGSVEKAISGCGVTNSFVFFRNIPEDLQSIPNMIRFDSIYMKAEL
401 EKAFDGIFQIEKSSVSEVVYETG
Amino acid composition:
A (Ala)27 (6.4%)C (Cys)3 (0.7%)H (His)15 (3.5%)M (Met)12 (2.8%)T (Thr)19 (4.5%)
R (Arg)16 (3.8%)Q (Gln)14 (3.3%)I (Ile)36 (8.5%)F (Phe)31 (7.3%)W (Trp)4 (0.9%)
N (Asn)20 (4.7%)E (Glu)30 (7.1%)L (Leu)34 (8.0%)P (Pro)14 (3.3%)Y (Tyr)19 (4.5%)
D (Asp)29 (6.9%)G (Gly)31 (7.3%)K (Lys)29 (6.9%)S (Ser)22 (5.2%)V (Val)18 (4.3%)
Amino acid counting:Total number:423Positively charged (Arg + Lys):45 (10.6%)Negatively charged (Asp + Glu):59 (13.9%)
Biochemical parameters:Molecular mass [Da]:48459.2Theoretical pI:5.55Extinction coefficient at 280 nm [M-1 cm-1]:50310 / 50497.5
(all Cys red/ox)
Estimated half-life [h]:E. coli:>10S. cervisiae:>20Mammals:30
Codon usage (CAI):E. coli:good (0.74)S. cervisiae:good (0.75)Mammals:good (0.66)
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please read the description.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]