Difference between revisions of "Part:BBa K1159003"

Revision as of 20:37, 26 July 2013

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1159003 short</partinfo>
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+<table border="1" cellspacing="0"><tr><td colspan="20"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a></strong></td></tr><tr><td colspan="20"><strong>Nucleotide sequence:</strong> (underlined part of entry was translated, italic parts were added)<br>GTACACA<u>ATGCGTCGT...TTCGAAAAA</u>TAA</tr><tr><td colspan="20"><strong>Amino acid sequence:</strong><br><code>MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE<br>FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW<br>FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL<br>DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT<br>ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE<br>FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK</code><br><br></span></tr><tr><td colspan="10">BioBrick: <partinfo>BBa_K801060</partinfo></td><td colspan="10">Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).</td></tr><tr><td colspan="2">A (Ala)</td><td colspan="2">33 (5.83%)</td><td colspan="2">R (Arg)</td><td colspan="2">25 (4.42%)</td><td colspan="2">N (Asn)</td><td colspan="2">27 (4.77%)</td><td colspan="2">D (Asp)</td><td colspan="2">34 (6.01%)</td><td colspan="2">C (Cys)</td><td colspan="2">4 (0.71%)</td></tr><tr><td colspan="2">Q (Gln)</td><td colspan="2">24 (4.24%)</td><td colspan="2">E (Glu)</td><td colspan="2">48 (8.48%)</td><td colspan="2">G (Gly)</td><td colspan="2">29 (5.12%)</td><td colspan="2">H (His)</td><td colspan="2">18 (3.18%)</td><td colspan="2">I (Ile)</td><td colspan="2">39 (6.89%)</td></tr><tr><td colspan="2">L (Leu)</td><td colspan="2">64 (11.31%)</td><td colspan="2">K (Lys)</td><td colspan="2">36 (6.36%)</td><td colspan="2">M (Met)</td><td colspan="2">16 (2.83%)</td><td colspan="2">F (Phe)</td><td colspan="2">28 (4.95%)</td><td colspan="2">P (Pro)</td><td colspan="2">17 (3.00%)</td></tr><tr><td colspan="2">S (Ser)</td><td colspan="2">33 (5.83%)</td><td colspan="2">T (Thr)</td><td colspan="2">26 (4.59%)</td><td colspan="2">W (Trp)</td><td colspan="2">14 (2.47%)</td><td colspan="2">Y (Tyr)</td><td colspan="2">27 (4.77%)</td><td colspan="2">V (Val)</td><td colspan="2">24 (4.24%)</td></tr><tr><td colspan="2"><strong>Amino acid counting:</strong></td><td colspan="4">Total number of amino acids (aa):</td><td colspan="2">566</td><td colspan="4">Number of positively charged aa (Arg + Lys):</td><td colspan="2">61</td><td colspan="4">Number of negatively charged aa (Asp + Glu):</td><td colspan="2">82</td></tr><tr><td colspan="2"><strong>Biochemical parameters:</strong></td><td colspan="4">Molecular weight [Da]:</td><td colspan="2">66105.33</td><td colspan="4">Theoretical pI:</td><td colspan="2">5.38</td><td colspan="4">Extinction coefficient: [M^-1 cm^-1]</td><td colspan="2">117480 (all Cys as cystine), 117230 (no Cys as cystine)</td></tr><tr><td colspan="2"><strong>Estimated half-life:</strong></td><td colspan="4"><i>Mammals:</i></td><td colspan="2">30 hour</td><td colspan="4"><i>Yeast:</i></td><td colspan="2">>20 hour</td><td colspan="4"><i>E. coli</i>:</td><td colspan="2">>10 hour</td></tr><tr><td colspan="2"><strong>Codon usage:</strong> (CAI)</td><td colspan="4"><i>Mammals:</i></td><td colspan="2">0.68</td><td colspan="4"><i>Yeast:</i></td><td colspan="2">0.69</td><td colspan="4"><i>E. coli</i>:</td><td colspan="2">0.71</td></tr><tr><td colspan="3"><strong>RFC standard:</strong></td><td colspan="17">This is a RFC 10 BioBrick, nothing was added</td></tr><tr><td colspan="20"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table><br>
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 ===Usage and Biology===