Difference between revisions of "Part:BBa M36093:Design"
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===Source=== | ===Source=== | ||
| − | Vector is based off of pJ1201-03c plasmid developed by DNA2.0 | + | Vector is based off of pJ1201-03c plasmid developed by DNA2.0. |
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| + | Note: Original pJ1201-03c plasmid does not have a yeast Kozak sequence. It has been added to this vector; student designed yeast sensors being synthesized by DNA2.0 will have the yeast Kozak automatically added to the 3' end of the design. | ||
===References=== | ===References=== | ||
Latest revision as of 17:43, 18 June 2013
S. cerevisiae sensor test rig
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4296
Illegal XbaI site found at 5221 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4296 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4296 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4296
Illegal XbaI site found at 5221 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4296
Illegal XbaI site found at 5221
Illegal AgeI site found at 1827
Illegal AgeI site found at 3909 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
Has S. cerevisiae specific LEU selection and 2 um origin of replication allowing for 50-100 copies of the plasmid in yeast cells.
Source
Vector is based off of pJ1201-03c plasmid developed by DNA2.0.
Note: Original pJ1201-03c plasmid does not have a yeast Kozak sequence. It has been added to this vector; student designed yeast sensors being synthesized by DNA2.0 will have the yeast Kozak automatically added to the 3' end of the design.