Difference between revisions of "Part:BBa K1104999:Design"

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<partinfo>BBa_K1104528 short</partinfo>
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<partinfo>BBa_K1104999 short</partinfo>
  
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<partinfo>BBa_K1104528 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1104999 SequenceAndFeatures</partinfo>
  
  
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===Source===
 
===Source===
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Streptomyces plicatus
  
 
===References===
 
===References===

Latest revision as of 07:23, 10 June 2013


This coding can make E. coli produce Chitinase-63, and degrade chitin.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 14
    Illegal NgoMIV site found at 233
    Illegal NgoMIV site found at 1760
    Illegal NgoMIV site found at 1775
    Illegal NgoMIV site found at 1832
    Illegal NgoMIV site found at 2201
    Illegal AgeI site found at 821
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 502
    Illegal BsaI site found at 1343
    Illegal BsaI site found at 1925
    Illegal BsaI.rc site found at 1358


Design Notes

We insert this coding to make E.coli produce Chitinase-63, in order to damage the spore wall of our target --pathogenic fungi, and kill them.


Source

Streptomyces plicatus

References

  • Phillips W. Robbins, Charles Albright, and Barbara Benfield(1988),Cloning and Expression of a Streptomyces plicatus Chitinase (Chitinase-63) in Escherichia coli. Vol. 263, No. 1, Issue of January 5, 443-447.
  • REFERENCE 1 (sites)

Robbins,P.W., Overbye,K., Albright,C., Benfield,B. and Pero,J. (1992)Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus. Gene 111 (1), 69-76.

  • REFERENCE 2 (bases 1 to 2276)

Delic,I., Robbins,P. and Westpheling,J. (1992) Direct repeat sequences are implicated in the regulation of two Streptomyces chitinase promoters that are subject to carbon catabolite control. Proc. Natl. Acad. Sci. U.S.A. 89 (5), 1885-1889.