Difference between revisions of "Part:BBa M1000"
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<partinfo>BBa_M1000 short</partinfo> | <partinfo>BBa_M1000 short</partinfo> | ||
− | Left end of a linear | + | *Left end of a linear transposome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. |
− | This part, and the companion part M1001 provide Biobrick tools to construct Tn5 | + | *This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. |
− | The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions. | + | *The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions. |
Revision as of 04:13, 21 June 2006
Left mosaic end for Tn5 transpososome
- Left end of a linear transposome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG.
- This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome.
- The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]