Difference between revisions of "Part:BBa K812133"
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<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
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Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>
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</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>  and J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>). This plasmid express sfGFP into Xenopus: <br/>
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</br><br/>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>  and J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">K812031</a>). This plasmid express sfGFP into Xenopus: <br/>
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<img src="https://static.igem.org/mediawiki/2012/2/27/PCS2%2B_sfGFP_20%2621_09.png" alt="perdu" width="880px" /> <br/><br/>
 
<img src="https://static.igem.org/mediawiki/2012/2/27/PCS2%2B_sfGFP_20%2621_09.png" alt="perdu" width="880px" /> <br/><br/>

Latest revision as of 16:18, 10 May 2013

sfGFP with kozak sequence for eukaryotic expression


*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812031. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***


This part was created with the plasmid backbone BBa_K812000 and J04450 was replaced by sfGFP (BBa_K812031). This plasmid express sfGFP into Xenopus:
perdu

The expression of sfGFP is present only in one of the two embryo/tadpole. sfGFP is present only in few tissue as others reporters. sfGFP is expressed in tail's muscles and branchial basket for this tadpole. After injection of this plasmid into Xenopus it does not diffuse in the eggs, and the plasmid express sfGFP in few different tissues.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19