Difference between revisions of "Part:BBa K511826"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K511826 short</partinfo> | ||
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+ | This MammoBlock composite device produces both the N-Cadherin cell-cell adhesion compound and the yellow fluorescent protein EYFP when induced with Gal4 transactivator variants and otherwise produces both proteins at a low, basal (OFF) level. This generator is particularly well suited to the engineering of cellular patterns given the combination of adhesion and fluorescent readout. | ||
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+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
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+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K511826 SequenceAndFeatures</partinfo> | ||
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+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K511826 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 16:18, 10 May 2013
Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device
This MammoBlock composite device produces both the N-Cadherin cell-cell adhesion compound and the yellow fluorescent protein EYFP when induced with Gal4 transactivator variants and otherwise produces both proteins at a low, basal (OFF) level. This generator is particularly well suited to the engineering of cellular patterns given the combination of adhesion and fluorescent readout.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 126
Illegal SpeI site found at 2965
Illegal PstI site found at 529
Illegal PstI site found at 1630
Illegal PstI site found at 3241 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 126
Illegal NheI site found at 154
Illegal SpeI site found at 2965
Illegal PstI site found at 529
Illegal PstI site found at 1630
Illegal PstI site found at 3241 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 126
Illegal BamHI site found at 1162
Illegal XhoI site found at 83 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 126
Illegal SpeI site found at 2965
Illegal PstI site found at 529
Illegal PstI site found at 1630
Illegal PstI site found at 3241 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 126
Illegal SpeI site found at 2965
Illegal PstI site found at 529
Illegal PstI site found at 1630
Illegal PstI site found at 3241
Illegal NgoMIV site found at 1481
Illegal NgoMIV site found at 3007 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1516
Illegal BsaI.rc site found at 2073