Difference between revisions of "Part:BBa K511823"
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This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with Gal4 transactivator variants and otherwise produces mKate at a low, basal (OFF) level. | This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with Gal4 transactivator variants and otherwise produces mKate at a low, basal (OFF) level. | ||
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Another activator promoter pair we make use of is the Gal4-UAS system. Gal4VP16 is an activator capable of binding to UAS promoter site and activating expression of downstream genes. Here we characterize this interaction with a set of transfections. Cells were transfected with UAS:(mKate, eYFP-FF4, eBFP2, or H2B-citrine) and Hef1a:GV16. Controls lack the Hef1a:GV16 plasmid. The above graph displays mean fluoresence in the denoted channels. | Another activator promoter pair we make use of is the Gal4-UAS system. Gal4VP16 is an activator capable of binding to UAS promoter site and activating expression of downstream genes. Here we characterize this interaction with a set of transfections. Cells were transfected with UAS:(mKate, eYFP-FF4, eBFP2, or H2B-citrine) and Hef1a:GV16. Controls lack the Hef1a:GV16 plasmid. The above graph displays mean fluoresence in the denoted channels. | ||
Latest revision as of 16:18, 10 May 2013
Inducible Red Fluorescent Protein Generator (UAS-Gal4-mKate) MammoBlock Device
This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with Gal4 transactivator variants and otherwise produces mKate at a low, basal (OFF) level.
Another activator promoter pair we make use of is the Gal4-UAS system. Gal4VP16 is an activator capable of binding to UAS promoter site and activating expression of downstream genes. Here we characterize this interaction with a set of transfections. Cells were transfected with UAS:(mKate, eYFP-FF4, eBFP2, or H2B-citrine) and Hef1a:GV16. Controls lack the Hef1a:GV16 plasmid. The above graph displays mean fluoresence in the denoted channels.
As can be clearly seen, Hef1a:GV16 results in significant activation of the UAS promoter. This results in the observed increases in mean fluoresence compared to control populations. With the exception of UAS:eBFP2, we see more than 20-fold increase in mean fluorescence.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 126
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 126
Illegal NheI site found at 154 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 126
Illegal XhoI site found at 83 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 126
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 126
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 876
Illegal SapI.rc site found at 258