Difference between revisions of "Part:BBa K511803"

 
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Latest revision as of 16:18, 10 May 2013


Repressible Fluorescence/Repression Generator (Hef1a-LacO-AmCyan-miRFF4) MammoBlock Device

This MammoBlock composite device produces the cyan fluorescent protein AmCyan-miRFF4 at a low, constitutive level; in addition, this device produces the inhibitory FF4 miRNA and can be inhibited by LacI variants.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal PstI site found at 1350
    Illegal PstI site found at 1427
    Illegal PstI site found at 2270
    Illegal PstI site found at 2693
    Illegal PstI site found at 3370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal PstI site found at 1350
    Illegal PstI site found at 1427
    Illegal PstI site found at 2270
    Illegal PstI site found at 2693
    Illegal PstI site found at 3370
    Illegal NotI site found at 1759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal PstI site found at 1350
    Illegal PstI site found at 1427
    Illegal PstI site found at 2270
    Illegal PstI site found at 2693
    Illegal PstI site found at 3370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal PstI site found at 1350
    Illegal PstI site found at 1427
    Illegal PstI site found at 2270
    Illegal PstI site found at 2693
    Illegal PstI site found at 3370
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
    Illegal AgeI site found at 1786
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1298
    Illegal BsaI site found at 1738
    Illegal BsaI.rc site found at 3003
    Illegal BsaI.rc site found at 3175
    Illegal SapI site found at 2491
    Illegal SapI.rc site found at 3263