Difference between revisions of "Part:BBa K511701"
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__NOTOC__
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<partinfo>BBa_K511701 short</partinfo>
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This part encodes a fusion between the N1a catalytic proteolytic domain of the tobacco etch virus (TEV) and the human B-Arrestin-2 sequence. The N1a domain recognizes the amino acid sequence ENLYFQX (where X is a variable amino acid) with unparalleled specificity, and is typically used for the removal of tags made for protein purification. The B-Arrestin-2 protein induces the extinction of nonvisual G-coupled protein receptor (GPCR) signaling in mammalian cells by binding to the cytoplasmic tails of induced GPCRs. This part is capable of cleaving GPCR-TEVs-Transactivator fusion proteins and freeing the tethered transactivator to induce transcriptional changes in a cell's nucleus.  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K511701 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K511701 parameters</partinfo>
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<!-- -->

Latest revision as of 16:18, 10 May 2013


B-Arrestin-2-TEV-Protease MammoBlock

This part encodes a fusion between the N1a catalytic proteolytic domain of the tobacco etch virus (TEV) and the human B-Arrestin-2 sequence. The N1a domain recognizes the amino acid sequence ENLYFQX (where X is a variable amino acid) with unparalleled specificity, and is typically used for the removal of tags made for protein purification. The B-Arrestin-2 protein induces the extinction of nonvisual G-coupled protein receptor (GPCR) signaling in mammalian cells by binding to the cytoplasmic tails of induced GPCRs. This part is capable of cleaving GPCR-TEVs-Transactivator fusion proteins and freeing the tethered transactivator to induce transcriptional changes in a cell's nucleus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 604
    Illegal XhoI site found at 998
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 307
    Illegal PstI site found at 394
    Illegal PstI site found at 1450
    Illegal AgeI site found at 590
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 865
    Illegal BsaI site found at 1830