Difference between revisions of "Part:BBa K427004"
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__NOTOC__
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<partinfo>BBa_K427004 short</partinfo>
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Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
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===Usage and Biology===
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The activation of the promoter requires the holenzyme σ70, which normally binds to the -35 and -10 consensus sequences, the promoter has the -10 but does not have the -35 sequences instead it has another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzyme σ70 recognizes the promoter and transcription begins.
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This part has no special safety considerations.
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<!-- -->
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===<span class='h3bb'>Sequence and Features</span>===
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<partinfo>BBa_K427004 SequenceAndFeatures</partinfo>
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===References===
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Jiang, Y. a. (2008). Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K427004 parameters</partinfo>
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Latest revision as of 16:17, 10 May 2013

Pmom promoter of Mu bacteriophage

Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.

Usage and Biology

The activation of the promoter requires the holenzyme σ70, which normally binds to the -35 and -10 consensus sequences, the promoter has the -10 but does not have the -35 sequences instead it has another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzyme σ70 recognizes the promoter and transcription begins.

This part has no special safety considerations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Jiang, Y. a. (2008). Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.