Difference between revisions of "Part:BBa P1014:Design"

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*Acquired by PCR from pTEX5500ts, GenbankDQ208936, kind gift of Barbara E. Murray.
 
*Acquired by PCR from pTEX5500ts, GenbankDQ208936, kind gift of Barbara E. Murray.
 
*pTEX5500ts was, in turn was derived from Genbank AF016483.
 
*pTEX5500ts was, in turn was derived from Genbank AF016483.
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Appl Environ Microbiol. 2006 Jan;72(1):334-45. Related Articles, Links
 
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Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains.
 
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Nallapareddy SR, Singh KV, Murray BE.
 
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# A pmid=16391062
 
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# B pmid=9593155
 
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</biblio>
 
  
 
===References===
 
===References===

Revision as of 17:15, 19 June 2006


gentamicin resistance cassette aph(2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR primers
    • gtttcttcgaattcgcggccgcttctagag gagataggttatgcaagat,P1014-F
    • gtttcttcctgcagcggccgctactagta gattccggattctaaaaaaagg,P1014-R2 (after terminator)
    • gtttcttcctgcagcggccgctactagta ctatttaattttaatgcttctg,P1014-R3 (after stop codon)


  • XbaI site at 660 removed by mutating c->t which also improves codon usage.
    • gatgatttttacagggatattttagagaacgaaatctac,P1014-XF
    • gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR

Source

  • Novel gentamicin resistance gene
  • Acquired by PCR from pTEX5500ts, GenbankDQ208936, kind gift of Barbara E. Murray.
  • pTEX5500ts was, in turn was derived from Genbank AF016483.

References