Difference between revisions of "Part:BBa P1014:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
 +
*Novel gentamicin resistance gene
 +
*Acquired by PCR from pTEX5500ts, GenbankDQ208936, kind gift of Barbara E. Murray.
 +
*pTEX5500ts was, in turn was derived from Genbank AF016483.
  
−
DQ208936
+
Appl Environ Microbiol. 2006 Jan;72(1):334-45. Related Articles, Links
 +
 +
Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains.
 +
 
 +
Nallapareddy SR, Singh KV, Murray BE.
 +
# A pmid=16391062
 +
# B pmid=9593155
 +
</biblio>
  
 
===References===
 
===References===

Revision as of 17:14, 19 June 2006


gentamicin resistance cassette aph(2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR primers
    • gtttcttcgaattcgcggccgcttctagag gagataggttatgcaagat,P1014-F
    • gtttcttcctgcagcggccgctactagta gattccggattctaaaaaaagg,P1014-R2 (after terminator)
    • gtttcttcctgcagcggccgctactagta ctatttaattttaatgcttctg,P1014-R3 (after stop codon)


  • XbaI site at 660 removed by mutating c->t which also improves codon usage.
    • gatgatttttacagggatattttagagaacgaaatctac,P1014-XF
    • gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR

Source

  • Novel gentamicin resistance gene
  • Acquired by PCR from pTEX5500ts, GenbankDQ208936, kind gift of Barbara E. Murray.
  • pTEX5500ts was, in turn was derived from Genbank AF016483.

Appl Environ Microbiol. 2006 Jan;72(1):334-45. Related Articles, Links

Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains.

Nallapareddy SR, Singh KV, Murray BE.

  1. A pmid=16391062
  2. B pmid=9593155

</biblio>

References