Difference between revisions of "Part:BBa P1014:Design"

(→‎Design Notes)
(→‎Design Notes)
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*PCR primers
 
*PCR primers
 
**gtttcttcgaattcgcggccgcttctagag gagataggttatgcaagat,P1014-F
 
**gtttcttcgaattcgcggccgcttctagag gagataggttatgcaagat,P1014-F
−
**gtttcttcctgcagcggccgctactagtacctttttttagaatccggaatc,P1014-R
+
**gtttcttcctgcagcggccgctactagta gattccggattctaaaaaaagg,P1014-R2 (after terminator)
 +
**gtttcttcctgcagcggccgctactagta ctatttaattttaatgcttctg,P1014-R3 (after stop codon)
  
  

Revision as of 17:06, 19 June 2006


gentamicin resistance cassette aph(2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR primers
    • gtttcttcgaattcgcggccgcttctagag gagataggttatgcaagat,P1014-F
    • gtttcttcctgcagcggccgctactagta gattccggattctaaaaaaagg,P1014-R2 (after terminator)
    • gtttcttcctgcagcggccgctactagta ctatttaattttaatgcttctg,P1014-R3 (after stop codon)


  • XbaI site at 660 removed by mutating c->t which also improves codon usage.
    • gatgatttttacagggatattttagagaacgaaatctac,P1014-XF
    • gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR

Source

DQ208936

References