Difference between revisions of "Test2"

(→‎Luciferases)
 
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[[image:Icon_Sensors.png]]
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[[image:Icon_RNA.png ]]
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*Proteinsynthesis
 +
**Polyadenylation
 +
**Terminators
 +
 
 +
*Tags
 +
**His-Tag
 +
**Strep-tagII
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**HA-tag
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**GST-Tag
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*Secretionsignals
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 +
 
  
  
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|align='center' width=80px |{{Click || image=Icon_Odor.png | link=Odor |width=50px | height=50px }}
 
|align='center' width=80px |{{Click || image=Icon_Odor.png | link=Odor |width=50px | height=50px }}
 
|'''[[Odor|Odor production and sensing]]''': Parts that produce or sense odorants aer listet here.
 
|'''[[Odor|Odor production and sensing]]''': Parts that produce or sense odorants aer listet here.
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|-
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|align='center' width=80px |{{Click || image=Icon_RNA.png | link=RNA |width=50px | height=50px }}
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|'''[[RNA]]''': Parts used as biosensors are listed here.
 
|-
 
|-
 
|align='center' width=80px |{{Click || image=Icon_Sensors.png | link=Sensors |width=50px | height=50px }}
 
|align='center' width=80px |{{Click || image=Icon_Sensors.png | link=Sensors |width=50px | height=50px }}
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<br><br><br><br><br><br><br><br><br>
 
<br><br><br><br><br><br><br><br><br>
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=Fluorescent Proteins=
 
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[https://parts.igem.org/Featured_Parts:Fluorescent_proteins Featured Parts],
 
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[http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],
 
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[http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala]
 
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https://parts.igem.org/Part:BBa_K180009 mBanana
 
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{|cellspacing="0" border="1"
 
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|Name
 
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|Exitation [nm]
 
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|Emmission [nm]
 
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|Quant. yield
 
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|Molar extinct.
 
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|BioBrick RFC10
 
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|BioBrick RFC25
 
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|Reference
 
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|-
 
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|style="background:" |Cerulean
 
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|433
 
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|475
 
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|0.62
 
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|43000
 
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|<partinfo>BBa_E2020</partinfo>
 
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|
 
−
|
 
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|-
 
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|style="background:" |GFPmut3b
 
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|501
 
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|511
 
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|
 
−
|
 
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|<partinfo>BBa_E0040</partinfo>
 
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|
 
−
|
 
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|-
 
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|style="background:#EEEE00" |amilGFP
 
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|503
 
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|512
 
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|0.67
 
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|75200
 
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|<partinfo>BBa_K592010</partinfo>
 
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|
 
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|[http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
 
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|-
 
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|style="background:" |mVenus
 
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|515
 
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|528
 
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|0.57
 
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|92200
 
−
|
 
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|<Partinfo>BBa_I757008</partinfo>
 
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|
 
−
|-
 
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|style="background:" |mOrange
 
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|548
 
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|562
 
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|0.69
 
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|71000
 
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|<partinfo>BBa_E2050</partinfo>
 
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|
 
−
|
 
−
|-
 
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|style="background:" |mStrawbery
 
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|574
 
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|596
 
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|0.29
 
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|90000
 
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|<partinfo>BBa_K399001</partinfo>
 
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|
 
−
|
 
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|-
 
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|style="background:#8B2252" |mRFP1
 
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|584
 
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|607
 
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|0.25
 
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|50000
 
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|<partinfo>BBa_E1010</partinfo>
 
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|
 
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|
 
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|-
 
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|style="background:" |mCherry
 
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|587
 
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|610
 
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|0.22
 
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|72000
 
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|<partinfo>BBa_E2060</partinfo>
 
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|
 
−
|
 
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|-
 
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|style="background:#FF34B3" |eforRed
 
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|589
 
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|609
 
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|0.16
 
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|111300
 
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|<partinfo>BBa_K592012</partinfo>
 
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|
 
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|[http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
 
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|-
 
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|style="background:#66CDAA" |cjBlue
 
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|610
 
−
|
 
−
|
 
−
|
 
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|<partinfo>BBa_K592011</partinfo>
 
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|
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
 
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|-
 
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|style="background:#473C8B" |aeBlue
 
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|
 
−
|
 
−
|
 
−
|
 
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|<partinfo>BBa_K864401</partinfo>
 
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|
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
 
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|-
 
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|style="background:#551A8B" |amilCP
 
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|588
 
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|NA
 
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|NA
 
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|8760
 
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|<partinfo>BBa_K592009</partinfo>
 
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|
 
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|[http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]
 
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|}
 
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<br>
 
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=Luciferases=
 
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{|cellspacing="0" border="1"
 
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|Name
 
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|Organism
 
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|Substrate
 
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|pH optimum
 
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|Amino acids
 
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|Mol. mass [kDa]
 
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|BioBrick RFC10
 
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|BioBrick RFC25
 
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|Reference
 
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|-
 
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|Photinus luciferase
 
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|
 
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|
 
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|
 
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|550
 
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|60.6
 
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|<partinfo>BBa_I712019</partinfo>
 
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|
 
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|
 
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|-
 
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|Renilla luciferase
 
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|
 
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|
 
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|
 
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|311
 
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|36.0
 
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|<partinfo>BBa_J52008</partinfo>
 
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|
 
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|
 
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|-
 
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|Gaussia luciferase
 
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|
 
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|
 
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|
 
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|189
 
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|20.3
 
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|<partinfo>BBa_K509005</partinfo>
 
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|
 
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|
 
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|-
 
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|Nano Luciferase
 
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|
 
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|
 
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|
 
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|
 
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|
 
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|171
 
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|19.1
 
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|[http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]
 
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|}
 
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=Photoproteins=
 
−
(Aequorin, Obelin
 

Latest revision as of 14:54, 16 March 2013

Icon RNA.png


  • Proteinsynthesis
    • Polyadenylation
    • Terminators
  • Tags
    • His-Tag
    • Strep-tagII
    • HA-tag
    • GST-Tag
  • Secretionsignals



Biosafety: Parts improving biological safety, containment.
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here.
Biodegradation: Parts involved in the degradation of chemicals are listed here.
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria.
Cell death: Parts involved in killing cells.
Coliroid: Parts involved in taking a bacterial photograph.
Conjugation: Parts involved in DNA conjugation between bacteria.
DNA binding: Parts involved in xxx.
DNA recombination: Parts involved in DNA recombination are listet here.
Ions: Parts involved in sensing, transport and binding of different ions are listet in here.
Ligand Binding: Parts involved in binding chemical structures are listet here.
Light production and sensing: Parts involved in generation and sensing of light are listet here.
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here.
Odor production and sensing: Parts that produce or sense odorants aer listet here.
RNA: Parts used as biosensors are listed here.
Sensors: Parts used as biosensors are listed here.
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here..