Difference between revisions of "Light"

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[[Catalog|< Back to Catalog]]
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=Fluorescent Proteins=
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[https://parts.igem.org/Featured_Parts:Fluorescent_proteins Featured Parts],
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[http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],
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{|cellspacing="0" border="1"
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|Name
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|Exitation [nm]
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|Emmission [nm]
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|Quant. yield
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|Molar extinct.
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|BioBrick RFC10
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|BioBrick RFC25
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|Reference
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|-
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|style="background:" |Cerulean
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|433
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|475
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|0.62
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|43000
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|<partinfo>BBa_E2020</partinfo>
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|
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|
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|-
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|style="background:" |GFPmut3b
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|501
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|511
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|
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|
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|<partinfo>BBa_E0040</partinfo>
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|
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|
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|-
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|style="background:#EEEE00" |amilGFP
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|503
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|512
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|0.67
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|75200
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|<partinfo>BBa_K592010</partinfo>
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|
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|[http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
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|-
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|style="background:" |mVenus
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|515
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|528
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|0.57
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|92200
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|
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|<Partinfo>BBa_I757008</partinfo>
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|
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|-
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|style="background:" |mOrange
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|548
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|562
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|0.69
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|71000
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|<partinfo>BBa_E2050</partinfo>
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|
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|
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|-
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|style="background:" |mStrawbery
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|574
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|596
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|0.29
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|90000
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|<partinfo>BBa_K399001</partinfo>
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|
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|
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|-
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|style="background:#8B2252" |mRFP1
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|584
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|607
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|0.25
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|50000
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|<partinfo>BBa_E1010</partinfo>
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|
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|
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|-
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|style="background:" |mCherry
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|587
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|610
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|0.22
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|72000
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|<partinfo>BBa_E2060</partinfo>
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|
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|
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|-
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|style="background:#FF34B3" |eforRed
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|589
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|609
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|0.16
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|111300
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|<partinfo>BBa_K592012</partinfo>
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|
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|[http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
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|-
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|style="background:#66CDAA" |cjBlue
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|610
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|
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|
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|
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|<partinfo>BBa_K592011</partinfo>
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|
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|[http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
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|-
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|style="background:#473C8B" |aeBlue
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|
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|
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|
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|
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|<partinfo>BBa_K864401</partinfo>
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|
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|[http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
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|-
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|style="background:#551A8B" |amilCP
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|588
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|NA
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|NA
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|8760
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|<partinfo>BBa_K592009</partinfo>
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|
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|[http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]
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|}
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<br>
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=Luciferases=
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{|cellspacing="0" border="1"
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|Name
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|Organism
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|Substrate
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|pH optimum
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|Amino acids
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|Mol. mass [kDa]
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|Emmission [nm]
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|BioBrick RFC10
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|BioBrick RFC25
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|Reference
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|-
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|Photinus/Firefly luciferase
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|D-luciferin + ATP
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|
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|
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|550
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|60.6
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|578
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|<partinfo>BBa_I712019</partinfo>
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|
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|
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|-
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|Renilla luciferase
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|coelenterazine + O<sub>2</sub>
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|
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|
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|311
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|36.0
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|480
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|<partinfo>BBa_J52008</partinfo>
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|
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|
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|-
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|Gaussia luciferase
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|coelenterazine + O<sub>2</sub>
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|
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|
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|189
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|20.3
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|480
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|<partinfo>BBa_K509005</partinfo>
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|
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|
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|-
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|Nano luciferase
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|furimazine
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|
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|
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|171
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|19.1
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|460
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|[http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]
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|
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|
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|}
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=Photoproteins=
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Aequorin, Obelin
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=Split-BioBricks for protein interactions=
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 +
=Degradation Tags for kinetic measurements=

Latest revision as of 12:22, 7 March 2013

< Back to Catalog


Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],


Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]


Luciferases

Name Organism Substrate pH optimum Amino acids Mol. mass [kDa] Emmission [nm] BioBrick RFC10 BioBrick RFC25 Reference
Photinus/Firefly luciferase D-luciferin + ATP 550 60.6 578 BBa_I712019
Renilla luciferase coelenterazine + O2 311 36.0 480 BBa_J52008
Gaussia luciferase coelenterazine + O2 189 20.3 480 BBa_K509005
Nano luciferase furimazine 171 19.1 460 [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]

Photoproteins

Aequorin, Obelin

Split-BioBricks for protein interactions

Degradation Tags for kinetic measurements