Difference between revisions of "Test2"
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|[http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1] | |[http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1] | ||
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=Photoproteins= | =Photoproteins= | ||
(Aequorin, Obelin | (Aequorin, Obelin |
Revision as of 22:29, 6 March 2013
Biosafety: Parts improving biological safety, containment. | |
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here. | |
Biodegradation: Parts involved in the degradation of chemicals are listed here. | |
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria. | |
Cell death: Parts involved in killing cells. | |
Coliroid: Parts involved in taking a bacterial photograph. | |
Conjugation: Parts involved in DNA conjugation between bacteria. | |
DNA binding: Parts involved in xxx. | |
DNA recombination: Parts involved in DNA recombination are listet here. | |
Ions: Parts involved in sensing, transport and binding of different ions are listet in here. | |
Ligand Binding: Parts involved in binding chemical structures are listet here. | |
Light production and sensing: Parts involved in generation and sensing of light are listet here. | |
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here. | |
Odor production and sensing: Parts that produce or sense odorants aer listet here. | |
Sensors: Parts used as biosensors are listed here. | |
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here.. |
Fluorescent Proteins
Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon], [http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala] https://parts.igem.org/Part:BBa_K180009 mBanana
Name | Exitation [nm] | Emmission [nm] | Quant. yield | Molar extinct. | BioBrick RFC10 | BioBrick RFC25 | Reference |
Cerulean | 433 | 475 | 0.62 | 43000 | BBa_E2020 | ||
GFPmut3b | 501 | 511 | BBa_E0040 | ||||
amilGFP | 503 | 512 | 0.67 | 75200 | BBa_K592010 | [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank] | |
mVenus | 515 | 528 | 0.57 | 92200 | BBa_I757008 | ||
mOrange | 548 | 562 | 0.69 | 71000 | BBa_E2050 | ||
mStrawbery | 574 | 596 | 0.29 | 90000 | BBa_K399001 | ||
mRFP1 | 584 | 607 | 0.25 | 50000 | BBa_E1010 | ||
mCherry | 587 | 610 | 0.22 | 72000 | BBa_E2060 | ||
eforRed | 589 | 609 | 0.16 | 111300 | BBa_K592012 | [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank] | |
cjBlue | 610 | BBa_K592011 | [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed] | ||||
aeBlue | BBa_K864401 | [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed] | |||||
amilCP | 588 | NA | NA | 8760 | BBa_K592009 | [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank] |
Luciferases
Name | Organism | Substrate | pH optimum | Amino acids | Mol. mass [kDa] | Emmission [nm] | BioBrick RFC10 | BioBrick RFC25 | Reference |
Photinus/Firefly luciferase | D-luciferin + ATP | 550 | 60.6 | 578 | BBa_I712019 | ||||
Renilla luciferase | coelenterazine + O2 | 311 | 36.0 | 480 | BBa_J52008 | ||||
Gaussia luciferase | coelenterazine + O2 | 189 | 20.3 | 480 | BBa_K509005 | ||||
Nano luciferase | furimazine | 171 | 19.1 | 460 | [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1] |
Photoproteins
(Aequorin, Obelin