Difference between revisions of "Part:BBa M36072:Experience"

Revision as of 22:31, 7 December 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36072

Our BIOE 44 lab group utilized this actuator attempting to drive its expression in e.coli. We attached the actuator to a rhamose sensor and attempted to over-express the protein. Unfortunately, we did not get our desired results. Our induced SoDA cells did were unable to increase cell viability when exposed to menadione versus the negative controls and our non-induced proteins.

User Reviews

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 ===Applications of BBa_M36072===
-Our BIOE 44 lab group utilized our actuator by expressing it in e.coli. We over expressed the superoxide free radical using varying levels of menadione. Unfortunately, we did not get our desired results. We attached our a rhamnose inducible sensor to our actuator. Our induced SoDA cells did not significantly increase survivability versus negative controls and our non-induced proteins.
+Our BIOE 44 lab group utilized this actuator attempting to drive its expression in e.coli. We attached the actuator  to a rhamose sensor and attempted to over-express the protein.  Unfortunately, we did not get our desired results. Our induced SoDA cells did were unable to increase cell viability when exposed to menadione versus the negative controls and our non-induced proteins.
 ===User Reviews===