Difference between revisions of "Part:BBa K560016"
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The backbone for golden gate assembly. | The backbone for golden gate assembly. | ||
| − | This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(BBa_K560012, BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal. | + | This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors([https://parts.igem.org/wiki/index.php?title=Part:BBa_K560012 BBa_K560012], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K560013 BBa_K560013], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K560014 BBa_K560014], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K560014 BBa_K560015]), and add adequate amount of ligase and Bsal. |
More details about experiment protocol can be found in our wiki. | More details about experiment protocol can be found in our wiki. | ||
| − | Details about the structure of this plasmid can be found in http://www.addgene. | + | Details about the structure of this plasmid can be found in http://www.addgene.org/31021/. |
| − | ==The | + | ==The Genetic Map== |
[[Image:PFUS_B4-giraffe-analyze-.png]] | [[Image:PFUS_B4-giraffe-analyze-.png]] | ||
Latest revision as of 16:38, 14 November 2012
BsaI Golden Gate Assembly_Backbone
The backbone for golden gate assembly. This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(BBa_K560012, BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal. More details about experiment protocol can be found in our wiki. Details about the structure of this plasmid can be found in http://www.addgene.org/31021/.
The Genetic Map
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 35
Illegal NheI site found at 2776 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XhoI site found at 363
Illegal XhoI site found at 1797 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644
Illegal NgoMIV site found at 1650
Illegal AgeI site found at 569 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 575
Illegal BsaI.rc site found at 128
Illegal SapI.rc site found at 2653