Difference between revisions of "Part:BBa K747100:Design"

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see for further information: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K747099 Eukaryotic TAL expression plasmid]
 
see for further information: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K747099 Eukaryotic TAL expression plasmid]
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The FokI nuclease was synthesized by IDT.
  
 
===References===
 
===References===

Revision as of 09:43, 31 October 2012

pTALEN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 966
    Illegal suffix found in sequence at 4462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 966
    Illegal SpeI site found at 4463
    Illegal PstI site found at 4477
    Illegal NotI site found at 972
    Illegal NotI site found at 2146
    Illegal NotI site found at 4470
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 966
    Illegal BglII site found at 1563
    Illegal BglII site found at 2914
    Illegal BamHI site found at 2217
    Illegal BamHI site found at 2920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 966
    Illegal suffix found in sequence at 4463
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 966
    Illegal XbaI site found at 981
    Illegal SpeI site found at 4463
    Illegal PstI site found at 4477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

see main page

Source

see for further information: Eukaryotic TAL expression plasmid

The FokI nuclease was synthesized by IDT.

References

1. Scholze, H. & Boch, J. TAL effectors are remote controls for gene activation. Current Opinion in Microbiology 14, 47–53 (2011).

2. Moscou, M. J. & Bogdanove, A. J. A Simple Cipher Governs DNA Recognition by TAL Effectors. Science 326, 1501–1501 (2009).

3. Cermak, T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 39, e82 (2011).

4. Reyon, D. et al. FLASH assembly of TALENs for high-throughput genome editing. Nature Biotechnology 30, 460–465 (2012).

5. Zhang, F. et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nature biotechnology 29, 149–153 (2011).

6. Miller, J. C. et al. A TALE nuclease architecture for efficient genome editing. Nature Biotechnology 29, 143–148 (2010).

7. Boch, J. et al. Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors. Science 326, 1509–1512 (2009).

8. Liu, J. et al. Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy. Journal of Genetics and Genomics 39, 209–215 (2012).

9. Wood, A. J. et al. Targeted Genome Editing Across Species Using ZFNs and TALENs. Science 333, 307–307 (2011).

10. Sander, J. D. et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol 29, 697–698 (2011).

11. Tesson, L. et al. Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology 29, 695–696 (2011).

12. Hockemeyer, D. et al. Genetic engineering of human pluripotent cells using TALE nucleases. Nature Biotechnology 29, 731–734 (2011) 13. Sanjana, N. E. et al. A transcription activator-like effector toolbox for genome engineering. Nature Protocols 7, 171–192 (2012).