Difference between revisions of "Part:BBa K917012:Experience"

Latest revision as of 11:44, 30 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917012

We have tested the expression of NapC protein under control of lac promoter in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and napC gene). Cell pellets were scanned prior to sonication and napC transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.

Haem staining results suggest NapC expression in E coli transformed with plac-napC and plac-ccm. The additional band indicated by an arrow in lane 7 represents NapC protein.

User Reviews

UNIQ0e74f8fa2dfb86d2-partinfo-00000000-QINU UNIQ0e74f8fa2dfb86d2-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
@@ Line 5: / Line 4: @@
 ===Applications of BBa_K917012===
+We have tested the expression of NapC protein under control of lac promoter in ''ccm'' transformed cells (''ccm'' gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ''ccm'' and ''napC'' gene). Cell pellets were scanned prior to sonication and ''napC'' transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.
+[[Image:Cytochrome compare.jpg]]
+Haem staining results suggest NapC expression in E coli transformed with ''plac-napC'' and ''plac-ccm''. The additional band indicated by an arrow in lane 7 represents NapC protein.
+[[Image:Gel_haem_compare_.jpg]]
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K917012 StartReviews</partinfo>