Difference between revisions of "Part:BBa K917009:Experience"
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K917009=== | ===Applications of BBa_K917009=== | ||
| + | We have tested the expression of CymA protein under control of lac promoter in ''ccm'' transformed cells (''ccm'' gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ''ccm'' and ''cymA'' gene). Cell pellets were scanned prior to sonication and ''cymA'' transformed cells show slightly more intense colour, indicating higher concentration of haem in cells. | ||
| + | [[Image:Cytochrome compare.jpg]] | ||
| + | Haem staining results suggest CymA expression in E coli transformed with ''plac-cymA'' and ''plac-ccm''. The additional band indicated by an arrow in lane 3 represents CymA protein. | ||
| + | [[Image:Gel_haem_compare_.jpg]] | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K917009 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K917009 StartReviews</partinfo> | ||
Latest revision as of 11:42, 30 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K917009
We have tested the expression of CymA protein under control of lac promoter in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and cymA gene). Cell pellets were scanned prior to sonication and cymA transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.
Haem staining results suggest CymA expression in E coli transformed with plac-cymA and plac-ccm. The additional band indicated by an arrow in lane 3 represents CymA protein.
User Reviews
UNIQa3eef15b3a73cf10-partinfo-00000000-QINU UNIQa3eef15b3a73cf10-partinfo-00000001-QINU