Difference between revisions of "Part:BBa K902017"
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| − | <html> This part was built to characterize the control element of the magnesium killswitch with mgtAp-mgtArb. GFP is used as a reporter to indicate how well the system responds to different concentrations of magnesium compared to controls. For the protocol and experimental design please click <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">here</a>. For characterization data please click <a href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">here</a>. This part shows that with lower concentrations of magnesium it is activated and with higher concentrations of magnesium it is repressed. </html>[[Image:Magmesium graph.png|thumb|center|500px|Figure 1: The blue line indicating the response to magnesium shows a dramatic decrease at approximately 1 mM concentration of magnesium. the system completely shuts off at 1mM magnesium.]] <html> It is important to note that these constructs do not contain the signal transduction components <a href="https://parts.igem.org/BBa_K902010">PhoP | + | <html> This part was built to characterize the control element of the magnesium killswitch with mgtAp-mgtArb. GFP is used as a reporter to indicate how well the system responds to different concentrations of magnesium compared to controls. For the protocol and experimental design please click <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">here</a>. For characterization data please click <a href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">here</a>. This part shows that with lower concentrations of magnesium it is activated and with higher concentrations of magnesium it is repressed. </html>[[Image:Magmesium graph.png|thumb|center|500px|Figure 1: The blue line indicating the response to magnesium shows a dramatic decrease at approximately 1 mM concentration of magnesium. the system completely shuts off at 1mM magnesium.]] <html> It is important to note that these constructs do not contain the signal transduction components <a href="https://parts.igem.org/BBa_K902010">PhoP</a> and <a href="https://parts.igem.org/BBa_K902011">PhoQ</a> and therefore the promoter riboswitch system appears to be leaky, i.e it does not completely shut off when there is 10mM magnesium. </html> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 09:34, 29 October 2012
MgtAp-mgtArb-GFP(LVA)
This part was built to characterize the control element of the magnesium killswitch with mgtAp-mgtArb. GFP is used as a reporter to indicate how well the system responds to different concentrations of magnesium compared to controls. For the protocol and experimental design please click here. For characterization data please click here. This part shows that with lower concentrations of magnesium it is activated and with higher concentrations of magnesium it is repressed. It is important to note that these constructs do not contain the signal transduction components PhoP and PhoQ and therefore the promoter riboswitch system appears to be leaky, i.e it does not completely shut off when there is 10mM magnesium.
Usage and Biology
The mgt system is used in the control and metabolism of magnesium in E. coli. It is important in regulating the transport of these ions into and out of the cell to ensure homeostasis is maintained. It has been previously characterized that the mgtA transport system contains an upstream promoter and riboswitch sensative to magnesium. We have cloned and biobricked both of these components and shown that they demonstrate the ability
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 974