Difference between revisions of "Part:BBa K902067"

Latest revision as of 22:18, 28 October 2012

Rhamnose promoter with S7 micrococal nuclease

NOTE: This was our original submission of the part. The S7 micrococcal nuclease contains a mutation at a single nucleotide and is thus ineffective. We have since resubmitted the part as K902084 in which we repaired the mutation. Please view the K902084 page and our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for details about the system.

This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K902067 short</partinfo>
-<html><p>This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose. </p></html>
+NOTE: This was our original submission of the part. The <i>S7 micrococcal nuclease</i> contains a mutation at a single nucleotide and is thus ineffective. We have since resubmitted the part as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902084 K902084] in which we repaired the mutation. Please view the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902084 K902084] page and our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for details about the system.
+This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===