Difference between revisions of "Part:BBa K902068"

Revision as of 20:38, 28 October 2012

RhaS

RhaS is a transcription factor which is part of the rhaSR operon.

Usage and Biology

RhaS activates expression of the rhaBAD operon on the P_rha rhamnose promoter. In order for it to bind to P_rha, the CRP-cAMP (cAMP receptor protein) complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).

In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).

The [2012.igem.org/Team:Calgary dddd] iGEM Calgary 2012 team] intended to upregulate rhaS so that they could use the rhamnose promoter to activate a kill switch without rhamnose necessarily being present in the environment. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details of the kill switch.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 440
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 172
1000
COMPATIBLE WITH RFC[1000]

Revision as of 20:36, 28 October 2012 (view source) Cmwinter (Talk \| contribs) ← Older edit		Revision as of 20:38, 28 October 2012 (view source) Cmwinter (Talk \| contribs) Newer edit →
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−	The [2012.igem.org/Team:Calgary iGEM Calgary 2012 team] intended to upregulate <i>rhaS</i> so that they could use the rhamnose promoter to activate a kill switch without rhamnose necessarily being present in the environment. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details of the kill switch.	+	The [2012.igem.org/Team:Calgary dddd] iGEM Calgary 2012 team] intended to upregulate <i>rhaS</i> so that they could use the rhamnose promoter to activate a kill switch without rhamnose necessarily being present in the environment. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details of the kill switch.