Difference between revisions of "Part:BBa K902065:Design"

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===Design Notes===
 
===Design Notes===
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The pRha promoter is two promoters in one. In its native E. coli, the 5' end of the promoter serves to express the rhaR and rhaS control genes. The 3' end of the promoter is expresses the rhaBAD rhamnose metabolism genes.
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This part was inserted into pSB1C3 using Biobrick Assembly Standards. Note that this promoter is double ended; thus designers must use caution in adding genes to the 5' end of the promoter. The 5' end is intended to express <i>rhaR</i> and <i>rhaS</i> regulatory proteins in native <i>E. coli</i>. The 3' end expresses the <i>rhaBAD</i> operon for rhamnose metabolism is controlled by the regulatory proteins on the opposite side of the promoter.
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The rhamnose response: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR] transcription factor is activated by rhamnose to upregulate [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 rhaR] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902068 rhaS]. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902068 RhaS] in turn up-regulates the rhaBAD operon. These functions are dependent on the binding of the catabolite receptor protein (CRP) cAMP complex to the promoter. Additionally, function of RhaS is improved in the presence of rhamnose (Egan & Schleif, 1993).
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The glucose response: The promoter is subject to repression via global catabolite repression.
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Please see the details on our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki].
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===Source===
 
===Source===

Latest revision as of 19:47, 28 October 2012

Rhamnose inducible, glucose repressible promoter (pRha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was inserted into pSB1C3 using Biobrick Assembly Standards. Note that this promoter is double ended; thus designers must use caution in adding genes to the 5' end of the promoter. The 5' end is intended to express rhaR and rhaS regulatory proteins in native E. coli. The 3' end expresses the rhaBAD operon for rhamnose metabolism is controlled by the regulatory proteins on the opposite side of the promoter.

Source

This promoter was commercially synthesized from the sequence characterized by Jeske and Altenbuchner (2010).

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.

Jeske, M., & Altenbuchner, J. (2010). The escherichia coli rhamnose promoter rhaP BAD is in pseudomonas putida KT2440 independent of crp--cAMP activation. Applied Microbiology and Biotechnology, 85(6), 1923-1933.