Difference between revisions of "Part:BBa K792003"

Latest revision as of 20:17, 27 October 2012

Import enhancer - HIV TAT penetratin (trojan peptide)

Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Jones et al 2005]. They can be used to increase the efficiency with which a protein enters a cell.

This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.

This part includes a HindIII restriction site as an extra.

See also:

Another Trojan Peptide part BBa_K792004

Trojan peptides can penetrate cell membranes. Extreme your safety measures when dealing with strains expressing this part: avoid direct contact with them or conditioned medium and discard them as pathogenic residues.

This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K792003 short</partinfo>
-Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.
+Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process ''[Jones et al 2005]''. They '''can be used to increase the efficiency with which a protein enters a cell'''.
+This part is the '''penetratin from the HIV TAT''' protein, and has to be used just before the coding sequence of the peptide that you want to be ''import enhanced''.
+This part includes a '''HindIII''' restriction site as an extra.
+{| style="width:100%"
+|-
+| style="background: yellow; width:11%" rowspan="2"| '''See also: '''
+| style="background: #D8D8D8" | Another Trojan Peptide part <partinfo>K792004</partinfo>
+|}
+{| style="width:100%; background: #FFBF00"
+|-
+| [[Image:BsAs2012-Biohazard.png | 70px]]
+| Trojan peptides can penetrate cell membranes. Extreme your safety measures when dealing with strains expressing this part: '''avoid direct contact with them or conditioned medium and discard them as pathogenic residues.'''
+|}
+{| style="width:100%; background: #CEE3F6"
+|-
+|This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a ''cross-feeding'' regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.
+|[[Image:Igem.bsas.png | 100px]]
+|}
-This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be ''import enhanced''.
 <!-- Add more about the biology of this part here