Difference between revisions of "Part:BBa K902078:Design"

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===Design Notes===
 
===Design Notes===
  
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This circuit was constructed from basic parts using the 3A assembly method. The S7 is toxic to cells and therefore these need to be grown up in media that does not contain any manganese.
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This circuit was constructed from basic parts using the BioBricks assembly method. The S7 is toxic to cells and therefore these need to be grown up in media that does not contain any manganese.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.

Latest revision as of 03:44, 27 October 2012

mntP promoter-mntP riboswitch-s7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 327


Design Notes

This circuit was constructed from basic parts using the BioBricks assembly method. The S7 is toxic to cells and therefore these need to be grown up in media that does not contain any manganese.

Source

S7 was obtained from S. aureus. This gene was synthesized from IDT.

References

Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.