Difference between revisions of "Part:BBa K902078:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This circuit was constructed from basic parts using the | + | This circuit was constructed from basic parts using the BioBricks assembly method. The S7 is toxic to cells and therefore these need to be grown up in media that does not contain any manganese. |
===Source=== | ===Source=== | ||
− | + | <html>S7 was obtained from <i>S. aureus</i>. This gene was synthesized from IDT.</html> | |
− | + | ||
===References=== | ===References=== | ||
+ | Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673. |
Latest revision as of 03:44, 27 October 2012
mntP promoter-mntP riboswitch-s7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 327
Design Notes
This circuit was constructed from basic parts using the BioBricks assembly method. The S7 is toxic to cells and therefore these need to be grown up in media that does not contain any manganese.
Source
S7 was obtained from S. aureus. This gene was synthesized from IDT.
References
Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.