Difference between revisions of "Part:BBa K902067:Design"

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===Source===
 
===Source===
  
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The [https://parts.igem.org/Part:BBa_K902065 pRha] promoter and [https://parts.igem.org/Part:BBa_K902019 S7] nuclease were commercially synthesized. The RBS was taken from the Parts Registry.
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The [https://parts.igem.org/Part:BBa_K902065 pRha] promoter and [https://parts.igem.org/Part:BBa_K902019 S7] nuclease were commercially synthesized. The RBS was taken from the Parts Registry. This part was constructed using the basic biobrick assembly method.
  
 
===References===
 
===References===
  
 
Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.
 
Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.

Revision as of 03:27, 27 October 2012

Rhamnose promoter with S7 micrococal nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed as part of a kill switch for 2012 iGEM Calgary project. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for more details about our kill system. This circuit needs to be grown up on special LB plates containing >0.2% glucose to repress this system successfully.

Source

The pRha promoter and S7 nuclease were commercially synthesized. The RBS was taken from the Parts Registry. This part was constructed using the basic biobrick assembly method.

References

Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.