Difference between revisions of "Part:BBa K818400"
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This is a coding construct for ''B. subtilis'' to produce AmilCP pigment in response to rotten meat volatiles in the environment. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012. | This is a coding construct for ''B. subtilis'' to produce AmilCP pigment in response to rotten meat volatiles in the environment. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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'''Expression in <i>E. coli</i>''' | '''Expression in <i>E. coli</i>''' | ||
SboA-AmilCP is strongly expressed in <i>E. coli</i>, on plate and in liquid culture, at normal growth conditions. On plate, the blue-purple colour is less visible compared to the cell pellet in liquid culture. | SboA-AmilCP is strongly expressed in <i>E. coli</i>, on plate and in liquid culture, at normal growth conditions. On plate, the blue-purple colour is less visible compared to the cell pellet in liquid culture. | ||
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'''Expression in <i>B. subtilis</i>''' | '''Expression in <i>B. subtilis</i>''' | ||
| − | SboA-AmilGFP was shown to be very weakly expressed in <i>Bacillus subtilis</i> on LB plate without rotten meat induction (faint color formation after | + | SboA-AmilGFP was shown to be very weakly expressed in <i>Bacillus subtilis</i> on LB plate without rotten meat induction (faint color formation after 4 days). This is probably due to the leakiness of the promoter. |
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| + | We (iGEM Groningen 2012) tested the expression of SboA-AmilCP in <i>Bacillus subtilis</i> subjected to volatiles from spoiled meat using the same setup as we used to test the sticker design (see our sticker page at http://2012.igem.org/Team:Groningen/Sticker). After this setup, a clear blue-purple color was visible. | ||
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| + | <!-- Add more about the biology of this part here | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 02:44, 27 October 2012
AmilCP with sboA regulator
This is a coding construct for B. subtilis to produce AmilCP pigment in response to rotten meat volatiles in the environment. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.
Usage and Biology
Expression in E. coli SboA-AmilCP is strongly expressed in E. coli, on plate and in liquid culture, at normal growth conditions. On plate, the blue-purple colour is less visible compared to the cell pellet in liquid culture.
Expression in B. subtilis
SboA-AmilGFP was shown to be very weakly expressed in Bacillus subtilis on LB plate without rotten meat induction (faint color formation after 4 days). This is probably due to the leakiness of the promoter.
We (iGEM Groningen 2012) tested the expression of SboA-AmilCP in Bacillus subtilis subjected to volatiles from spoiled meat using the same setup as we used to test the sticker design (see our sticker page at http://2012.igem.org/Team:Groningen/Sticker). After this setup, a clear blue-purple color was visible.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]