Difference between revisions of "Part:BBa K902054:Design"

 
(→‎Source)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K902054 short</partinfo>
 
<partinfo>BBa_K902054 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry.
+
DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry. This part was constructed using the basic biobrick assembly method.
  
 
===References===
 
===References===
 +
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21

Latest revision as of 02:32, 27 October 2012

Inducible DszB generator (J04500-DszB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 652
    Illegal XhoI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 359
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See DszB mutations


Source

DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry. This part was constructed using the basic biobrick assembly method.

References

Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21