Difference between revisions of "Part:BBa K902028:Design"

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===Design Notes===
 
===Design Notes===
−
There are no illegal cut sites. This part was constructed with the Moco riboswitch and the CviaII restriction endonuclease using the BioBrick Assembly method.
+
There are no illegal cut sites.  
  
 
===Source===
 
===Source===
  
−
moco-cviaii
+
The Moco-CviaII composite part. This part was constructed with the Moco riboswitch and the CviaII restriction endonuclease using the BioBrick Assembly method.
  
 
===References===
 
===References===
 
1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932
 
1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932
 +
 +
2) Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucl. Acids Res. (1986) 14 (15): 6017-6030.

Latest revision as of 01:54, 27 October 2012

Moco Riboswitch-CviAII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BglII site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no illegal cut sites.

Source

The Moco-CviaII composite part. This part was constructed with the Moco riboswitch and the CviaII restriction endonuclease using the BioBrick Assembly method.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932

2) Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucl. Acids Res. (1986) 14 (15): 6017-6030.