Difference between revisions of "Part:BBa K902027:Design"

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===Design Notes===
 
===Design Notes===
LacI(R0010)-MOCO riboswitch-CviAII
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There are no illegal cut sites.
 
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===Source===
 
===Source===
 
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The CviaII and the Moco riboswtich were synthesized through IDT. The sequence was derived from the NCBI Gene Database. This brick was constructed using the biobrick assembly method.
LacI(R0010)-MOCO riboswitch-CviAII
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===References===
 
===References===

Latest revision as of 01:49, 27 October 2012

LacI(R0010)-MOCO riboswitch-CviAII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no illegal cut sites.

Source

The CviaII and the Moco riboswtich were synthesized through IDT. The sequence was derived from the NCBI Gene Database. This brick was constructed using the biobrick assembly method.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932

2) Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucl. Acids Res. (1986) 14 (15): 6017-6030.