Difference between revisions of "Part:BBa K902025:Design"

Latest revision as of 01:45, 27 October 2012

Moco Riboswitch- GFP(LVA)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 779

Design Notes

There are no illegal cut sites. Changes to the DNA sequence can inactivate the brick through changes in the RNA secondary structure.

Source

The Moco Riboswitch was synthesized from IDT. The GFP(LVA) part was used from the registry parts. This brick was assembled using the biobrick assembly method.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932

@@ Line 6: / Line 6: @@
 ===Design Notes===
-The brick was assembled using the biobrick assembly method and has no illegal cut sites. Changes to the DNA sequence can inactivate the brick through changes in the RNA secondary structure.
+There are no illegal cut sites. Changes to the DNA sequence can inactivate the brick through changes in the RNA secondary structure.
 ===Source===
-The Moco Riboswitch was synthesized from IDT. The GFP(LVA) part was used from the registry parts.
+The Moco Riboswitch was synthesized from IDT. The GFP(LVA) part was used from the registry parts. This brick was assembled using the biobrick assembly method.
 ===References===
 ) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932