Difference between revisions of "Part:BBa K902080:Design"
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− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K902080 short</partinfo> | <partinfo>BBa_K902080 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | mutation | + | DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info |
+ | |||
+ | <i>dszB</i> and <i>dszC</i> were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info | ||
Line 13: | Line 14: | ||
===Source=== | ===Source=== | ||
− | + | Assembled from biobrick parts | |
===References=== | ===References=== | ||
+ | Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from <i>Rhodococcus erythropolis</i> KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21 |
Revision as of 23:42, 26 October 2012
PlacI/dszB/B0034/dszC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1428
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 652
Illegal BamHI site found at 2232
Illegal XhoI site found at 644 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 359
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2382
Design Notes
DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info
dszB and dszC were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info
Source
Assembled from biobrick parts
References
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21