Difference between revisions of "Part:BBa K902002"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K902002 short</partinfo>
 
<partinfo>BBa_K902002 short</partinfo>
  
This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.
+
This part contains the beta-glucuronidase (<i>uidA</i>) enzyme from E. coli under the control of an IPTG inducible promoter.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 
+
The <i>uidA</i> gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay.  It is also known as the <i>gusA</i> gene and has numerous homologous in various bacterial and plant species.  It has specific activity against glucuronoside linked to a variety of other components.  This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically.  See our experience page for more information on its characterization.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 21:48, 26 October 2012

Beta-glucuronidase (uidA) inducible generator

This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 732
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]