Difference between revisions of "Part:BBa K902002"
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<partinfo>BBa_K902002 short</partinfo> | <partinfo>BBa_K902002 short</partinfo> | ||
− | This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter. | + | This part contains the beta-glucuronidase (<i>uidA</i>) enzyme from E. coli under the control of an IPTG inducible promoter. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The <i>uidA</i> gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay. It is also known as the <i>gusA</i> gene and has numerous homologous in various bacterial and plant species. It has specific activity against glucuronoside linked to a variety of other components. This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically. See our experience page for more information on its characterization. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 21:48, 26 October 2012
Beta-glucuronidase (uidA) inducible generator
This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 732
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]