Difference between revisions of "Part:BBa K731040:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was created by Gibson assembly starting from K731030. sfGFP was amplified by PCR from | + | This part was created by Gibson assembly starting from K731030. sfGFP was amplified by PCR from K515005, removing the RBS. CysE and sfGFP are separated by a short nucleotide linker that encodes for the amino acidic sequence GSGSGS. |
===Source=== | ===Source=== | ||
| Line 13: | Line 13: | ||
===References=== | ===References=== | ||
| + | Jones-Mortimer, M.C.(1968). The control of sulphate reduction in Escherichia coli by O-acetyl-l-serine. Biochem. J. 106, 33. <br> | ||
| + | Kredich & Tomkins, 1966. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. Biochem. J. 241, 4955-4965. <br> | ||
| + | Denk, D., and A. Bock. 1987. L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant. J. Gen. Microbiol. 133:515–525. <br> | ||
Latest revision as of 21:29, 26 October 2012
Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 2045 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1909 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 2075
Design Notes
This part was created by Gibson assembly starting from K731030. sfGFP was amplified by PCR from K515005, removing the RBS. CysE and sfGFP are separated by a short nucleotide linker that encodes for the amino acidic sequence GSGSGS.
Source
K731030
References
Jones-Mortimer, M.C.(1968). The control of sulphate reduction in Escherichia coli by O-acetyl-l-serine. Biochem. J. 106, 33.
Kredich & Tomkins, 1966. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. Biochem. J. 241, 4955-4965.
Denk, D., and A. Bock. 1987. L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant. J. Gen. Microbiol. 133:515–525.